TABLE 4.
Cloning primers and E. coli expression vectors used in this study
| Gene | Vector | Forward primer (5′ → 3′)a | Reverse primer (5′ → 3′)a |
|---|---|---|---|
| BACOVA_02653 | pET21a | CTTTTATATCATGTAGCCATATGGATGAAAAAGGAGG | GGAGCTCTCGAGATTTAATCCTTTCATAATCGC |
| BACCELL_02631 | pMCSG53 | TACTTCCAATCCAATGCCATGGATCCTGTAACATCGGAAATAA | TTATCCACTTCCAATGTTATTATTGCGGTGCATTGTCATAGACG |
| BACUNI_00321 | pET28a | GGAGCTCATATGTGTTCCGGCAATGATGATAC | GGAGCTCTCGAGTCAATAAGGGTATTTGCCGGC |
| HMPREF9446_01793 | pET28a | GGAGCTCATATGTCGGATGACAATTCCGGTAC | GGAGCTCTCGAGTCAGTAAGGATACTTTCCGTCTC |
| HMPREF9455_00295 | pET28a | GGAGCTCATATGGGCGACGATGATGATCCGATA | GGAGCTCTCGAGTTAATTTCCTGCATCTATAATAGCACTGATAG |
| BACUNI_00326 | pET28a | GGAGCTCATATGAATCATCAGCTATACTATACATCG | GGAGCTCTCGAGCTATAAGAAATGTATGGTCACCGCCTC |
| HMPREF9446_01800 | pET28a | GGAGCTCATATGAACCACCGGCTTTATTATACCA | GGAGCTCTCGAGTCAAAAAGAAAAGAAAGTGA |
| HMPREF9455_00301 | pMCSG53 | TACTTCCAATCCAATGCCATGAAATGGCAATATTATTTCAACGA | TTATCCACTTCCAATGTTATTATAGAAATCTTGATATTCTGATT |
| BACUNI_00317 | pET28a-TEV | AAAAACATATGGGGGCAAAAACCTATGTTTCCG | AAAAACTCGAGTTATTCAATGGTCATCGAAATTATCTC |
| HMPREF9446_01791 | pET28a-TEV | AAAAACATATGGACAAGGGGCTGACCACTGATGAC | AAAAACTCGAGTTACAATACCTTCAGACTATCTATCG |
| HMPREF9455_00296 | pET28a-TEV | AAAAAGCTAGCGACAAATACGAGATGGGTGCAAGC | AAAAACTCGAGTTATTTTAGAATATAATGATAAATAG |
| BACUNI_00318 | pMCSG53 | TACTTCCAATCCAATGCCATGCACACCACCCCGATTCTGAACGAATC | TTATCCACTTCCAATGTTATTACCATTCAGGAACAGAGAAAAGAG |
a
Restriction sites are underlined, and pMCSG LIC vector complementary sequences are in boldface.