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Primers used for site-directed mutagenesis
| Gene | Residue change | Forward primer (5′ → 3′)a | Reverse primer (5′ → 3′)a |
|---|---|---|---|
| BACUNI_00326 | E531A | ACTGCCCCCACTTCTTCTCCGGCGAACAGCTTCTATGTGCCGGGT | ACCCGGCACATAGAAGCTGTTCGCCGGAGAAGAAGTGGGGGCAGT |
| HMPREF9446_01800 | E543A | ACCGCTCCCACTTCTTCACCGGCTAACAGTTTCTATGTGCCGGGC | GCCCGGCACATAGAAACTGTTAGCCGGTGAAGAAGTGGGAGCGGT |
Changed bases are underlined.
