FIG 6.

Volcano CbsR12 Crosslink-Seq plot highlights mRNAs that occur at different levels between Crosslink-Seq experiments with strains MB-WT and MB-cbsR12. CbsR12 targets several C. burnetii transcripts, including those of metK, carA, and cvpD. Labeled transcripts are indicated by green dots and are significantly enriched in MB-WT versus MB-cbsR12, identifying them as targets of CbsR12. Black dots represent transcripts not significantly different between the strains tested. Orange dots represent transcripts having a log₂ fold change of >1 but a false discovery rate (FDR) of >0.05. There were no transcripts indicated by red dots, which would represent mRNAs with an FDR of <0.05 but a log₂ fold change in levels of <1. Data shown are representative of two biological replicates each of MB-WT and MB-cbsR12 Crosslink-Seq experiments. The potential CbsR12-binding site in the coding region of the ahcY transcript is indicated in the inset.