Skip to main content
. 2019 Oct 21;201(22):e00524-19. doi: 10.1128/JB.00524-19

FIG 9.

FIG 9

CarA and MetK proteins are differentially synthesized in MB-WT, MB-cbsR12, and MB-cbsR12-Comp strains. (A) Proteins (30 μg total) from MB-WT, MB-cbsR12, and MB-cbsR12-Comp LCVs (mid-log phase; 96 h for MB-WT and MB-cbsR12-Comp and 144 h for MB-cbsR12) grown in ACCM-2 were resolved on a 10 to 20% acrylamide gradient SDS-PAGE gel, blotted, probed with rabbit anti-CarA antibodies, and detected with chemiluminescence. The arrow indicates CarA. (B) Proteins (60 μg total) from MB-WT, MB-cbsR12, and MB-cbsR12-Comp LCVs (mid-log phase; 96 h for MB-WT and MB-cbsR12-Comp and 144 h for MB-cbsR12) grown in ACCM-2 were resolved on 10 to 20% acrylamide gradient SDS-PAGE gels, blotted, probed with rabbit anti-MetK antibodies, and detected using chemiluminescence. The arrow indicates MetK.