TABLE 1.
Expression of mRNAs associated with anabolic activity and ATP metabolism is reduced in P. aeruginosa biofilms relative to that in exponential-phase planktonic culturesa
| Gene ontology term | P value |
|---|---|
| Structural constituent of ribosome | 1 × 10−24 |
| ATP binding | 2 × 10−10 |
| Biosynthesis of secondary metabolites | 7 × 10−5 |
| Pyridoxal phosphate binding | 0.0004 |
| Aminoacyl-tRNA biosynthesis | 0.0007 |
| Proton-transporting ATP synthase activity, rotational mechanism | 0.0007 |
| Oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor | 0.0016 |
| Histidine biosynthetic process | 0.0041 |
| Fatty acid biosynthetic process | 0.0104 |
| Peptidyl-prolyl cis-trans isomerase activity | 0.0111 |
| DNA topological change | 0.0132 |
| GTP binding | 0.0195 |
| Cobalamin/porphyrin biosynthetic process | 0.0221 |
| Arginine biosynthetic process | 0.0230 |
| Lysine biosynthetic process via diaminopimelate | 0.0285 |
| ATP synthesis coupled electron transport | 0.0304 |
| Pyridoxine biosynthetic process | 0.0350 |
a
Functional annotation clustering of P. aeruginosa genes downregulated in biofilms relative to planktonic growth (>2-fold change; P < 0.05) is based on gene enrichment analysis of gene ontology groups using DAVID. There were no statistically significant clusters for genes upregulated in biofilms using these parameters.