FIG 6.

Simultaneous modification of multiple lipoyl domain-containing proteins by LipL. Lipoyl transfer from LA-GcvH (5 μM) (lanes 1 and 2), LA-GcvH-L (20 μM) (lanes 3 and 4), LA-E2-PDH (5 μM) (lanes 5 and 6), LA-E2-OGDH (5 μM) (lanes 7 and 8), and LA-E2-BCODH (5 μM) (lanes 9 and 10) to the other four apo-lipoyl domain-containing proteins (10 μM of each) with LipL (2 μM) (even-numbered lanes) and without LipL (odd-numbered lanes) was assessed by immunoblotting with rabbit anti-lipoic acid antibody. Five microliters of a 100-μl reaction mixture was loaded into each lane. The presented blot is representative of at least three independent experiments.