FIG 5.

RNase E depletion decreases type III effector protein secretion. The effects of RNase E depletion on the production of T3SS regulators ExsA and ExsD, as well as production and export of a T3SS translocator protein (PopD), and the effector ExoT were monitored by Western blotting. Amounts of ExsA and ExsD, T3SS regulatory proteins, are also decreased under RNase E depletion conditions. RpoB was detected as a fractionation control. The ΔpscJ mutant strain does not assemble a functional T3SS. w.t., wild type.