TABLE 2.
Primers used in this study
| Name | Sequence | Description |
|---|---|---|
| rsmA5-1 | AAAAAgaattcCGACACCAACATCGCCAAGGTT | rsmA deletion, removing codons 3–59, outside primer, EcoRI site (lowercase) |
| rsmA5-2 | AACTCGAGCCGCAAGCATGCTGAACAGCATTCCTTTCTCCTCACGCGAA | Pair with rsmA5-1 |
| rsmA3-1 | TTCAGCATGCTTGCGGCTCGAGTTAACCATTAATTTTTATCTAATTTT | Pair with rsmA3-2 |
| rsmA3-2 | AAAAAaagcttTAACGCTTGTTTTACCGTGAAAGA | Outside primer, HindIII site (lowercase) |
| rsmA-Myc-5-2 | CTCCGAGATCAGTTTCTGCTCGCCCAAATCTTCTTCAGAAATCAACTTTTGTTCATGGTTTGGCTCTTGATCTTTCTC | Fuse 2× Myc tag to 3′ end of rsmA, pair with rsmA5-1 |
| rsmA-Myc-3-1 | GAAGAAGATTTGGGCGAGCAGAAACTGATCTCGGAGGAGGACCTGCAATGATTTTTATCTAATTTTCCCTTTGCA | Pair with rsmA3-2 |
| rsmV-5-1 | AAAAAtctagaATGGGTTTCCGGCCAGTTAGC | rsmV deletion 5′ flank outside primer, XbaI site (lowercase) |
| rsmV-5-2 | GGATAGCGGGAGGGGATGAGGTTGGAAATCTACCAAGC | Pair with rsmV-5-1 |
| rsmV-3-1 | GCTTGGTAGATTTCCAACCTCATCCCCTCCCGCTATCC | Pair with rsmV-3-2 |
| rsmV-3-2 | AAAAAaagcttCCGCTGGCGATCAGGTACAGCAC | rsmV deletion, 3′ flank outside primer, HindIII site (lowercase) |
| rsmW-5-1 | AAAAAtctagaGCCCTGGACTATACCGCCAAC | rsmW deletion 5′ flank outside primer, XbaI site (lowercase) |
| rsmW-5-2 | GGAAAGACCGGGACATGAGGGCTCAGCGCAGGGCGCGGGCGTAG | Pair with rsmW-5-1 |
| rsmW-3-1 | CTACGCCCGCGCCCTGCGCTGAGCCCTCATGTCCCGGTCTTTCC | Pair with rsmW-3-2 |
| rsmW-3-2 | AAAAAaagcttGGTCGAACTCTTCGAAGCTGTA | rsmW deletion, 3′ flank outside primer, HindIII site (lowercase) |
| delrsmY F1 | ATATggatccGGTGGCCACGTAGTTCGGGG | rsmY deletion 5′ flank outside primer, BamHI site (lowercase) |
| delrsmY R2 | GCGGTTTTCCTCGGGCAATAAGGTTTGAAGATTACGCATCTCTG | rsmY reverse inner primer |
| delrsmY F3 | TGCGTAATCTTCAAACCTTATTGCCCGAGGAAAACCGCGTCGCT | rsmY forward inner primer |
| delrsmY R4 | ATATctcgagCTGCTCACCGGCAACCTGGA | rsmY deletion 3′ flank outside primer, XhoI site (lowercase) |
| delrsmZ F1 | ATATggatccCGAGCTGCTGCAGGATGACG | rsmZ deletion 5′ flank outside primer, BamHI site (lowercase) |
| delrsmZ R2 | ACGAGTAAAACGGCAGGCAAACAGGAGTGATATTAGCGATTCC | rsmZ reverse inner primer |
| delrsmZ F3 | CGCTAATATCACTCCTGTTTGCCTGCCGTTTTACTCGTCGCCAA | rsmZ forward inner primer |
| delrsmZ R4 | ATATctcgagGCCCGCGGCAAGCTCTCGAT | rsmZ deletion 3′ flank outside primer, XhoI site (lowercase) |
| rneVDAS4-F1 | ATATggatccAGGACGAGCAGGACGATACCGATG | Forward primer to fuse VDAS4 tag to rne 3′ end, BamHI site (lowercase) |
| rneVDAS4-R2 | CCTAGGTCAGCTGGCGTCGGCGTAGTTCTCGCTGTA | rne reverse inner primer to fuse VDAS4 tag to rne 3′ end |
| rneVDAS4-F3 | GCCAGCTGACCTAGGGTGACAGGCTGAAAAAAGGG | rne forward inner primer to fuse VDAS4 tag to rne 3′ end |
| rneVDAS4-R4 | ATATgaattcCTGACCCGTGACGTCGAGGCGCTG | Reverse primer to fuse VDAS4 tag to rne 3′ end, EcoRI site (lowercase) |
| PA0077-RT F1 | ATCGACAGCCTGCTGGAAGACAT | PA0077 (icmF1) forward primer for real-time PCR |
| PA0077-RT R1 | TGGTGGAGTTGACCACGTTCTTCA | PA0077 (icmF1) reverse primer for real-time PCR |
| exsA-RT F1 | ATGCAAGGAGCCAAATCTCTTGG | exsA forward primer for real-time PCR |
| exsA-RT R1 | TCAGTTATTTTTAGCCCGGCATTC | exsA reverse primer for real-time PCR |