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T3SS-associated gene transcription, protein secretion profiles, and clearance of PA14 strains in infection model. (A) Reverse transcriptase quantitative PCR (RT-qPCR) analysis for T3SS-related genes across PA14 strains, normalized to WT PA14 RNA levels. (B) Immunoblot analysis of exoenzyme U and exoenzyme S secretion for PA14 strains grown in MinS medium (for type III protein secretion induction).
