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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Cell Microbiol. 2018 Dec 7;21(3):e12976. doi: 10.1111/cmi.12976

Figure 4 – Disruption of membrane rafts by m-β-CD decreases adhesion and internalization of Hc yeasts.

Figure 4 –

(A) J774.16 Mφs were treated, or not with 5, 10 or 20 mM of m-β-CD and then incubated with non-opsonized (left panels) or anti-Hsp60 mAb opsonized (right panels) yeast previously labeled with NHS-Rho. After 45 minutes of interaction, Uvitex 2B was added to stain extracellular fungi. Percentages of adherent and internalized yeasts are only for Mφs associated with Hc. The effect on sterol depletion by m-β-CD treatment was overcome by yeast opsonization at concentrations of 5 and 10 mM, and partially reversed at 20 mM m-β-CD. (B) Mφs treated with 10 mM of m-β-CD were also labeled with anti-CD11b or CD18 to confirm the levels of CR3 on the cell surface and no changes were detected. The experiments were performed 3 different times with similar results. Statistical analyses of these data are shown in Supplementary Table 2.