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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Cell Microbiol. 2018 Dec 7;21(3):e12976. doi: 10.1111/cmi.12976

Figure 6 – Complex gangliosides are required for correct CD18 recruitment to lipid microdomains.

Figure 6 –

(A) Lipid microdomains were isolated from WT and B4galnt1−/− mice Mφs. Fractions from sucrose gradients were probed with peroxidase-conjugated antibodies to CD14 or to CD18 (Becton Dickinson). Peroxidase-labeled CtxB (Sigma) was used to detect GM1. (B) Densitometric analysis of fractions corresponding to lipids microdomains was performed using ImageJ and the relative values from one experiment are presented. Experiments were performed three times with similar results (*p<0.05).