(A) GFP-Hc yeast cells were incubated for 5 minutes with WT, CD18low or CD11b−/− Mφs. The association levels were analyzed by flow cytometry (*p<0.001). (B) Bone marrow-derived murine Mφs were incubated with Uvitex 2B-labeled Hc for 15 minutes (moi 1:2), and the distribution of ganglioside GM1 (green), CD18 (red, upper panel) and CD11b (red, lower panel) were evaluated. The overlay images show the binding sites between Mφs and Hc by the colocalization of integrins and GM1. Bar, 10 μm.