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. 2019 Oct 22;8:e51109. doi: 10.7554/eLife.51109

Figure 4. ICP4 binds cellular promoters during early infection.

Figure 4.

MRC5 cells were infected with HSV-1 for 2, 4, or 6 h, and ChIP-Seq for ICP4 was performed. Data was aligned to the human genome (hg38) and normalized for cellular genome sampling. (A) Representative region of the cellular genome. ICP4 binding motifs are indicated in red. (B, D) Sequencing data centered + /- 1 kilobase from the transcription start site (TSS) of cellular mRNAs. (C) Sequencing data scaled from mRNA TSS to the transcription end site (TES). (E) Intersection of MACS2 identified ICP4 occupied regions. (F) Top 10 enriched Reactome groups for genes (n = 2190) with ICP4 bound at the promoter at 2 hr.