Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo-sapiens) | MRC5 | ATCC | ATCC CCL-171 RRID:CVCL_0440 |
|
| Cell line (Cercopithecus aethiops) | Vero | ATCC | ATCC CCL-81 RRID:CVCL_0059 |
|
| Cell line (Cercopithecus aethiops) | E5 | DeLuca and Schaffer, 1988 | E5 | Vero cells stably expressing ICP4 |
| Strain, strain background (Human herpesvirus 1) |
KOS | Smith, 1964 | ||
| Strain, strain background (Human herpesvirus 1) |
n12 | DeLuca and Schaffer, 1988 | Strain KOS with nonsense mutation near ICP4 N-terminus | |
| Strain, strain background (Human herpesvirus 1) |
tsKos | Dremel and DeLuca, 2019 | Strain KOS with temperature sensitive ICP4 | ICP4 has an A474V mutation |
| Antibody | Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) Mouse Monoclonal | Abcam | Cat #ab5408 | |
| Antibody | Anti-TATA binding protein Mouse Monoclonal |
Abcam | Cat #ab51841 | |
| Antibody | Anti-Sp1 Mouse Monoclonal |
Santa Cruz | Cat #sc-17824 | |
| Antibody | Anti-Human Sur-2 (Med23) Mouse Monoclonal |
BD Pharmingen | Cat #550429 | |
| Antibody | Anti-Med1 Rabbit Polyclonal |
Bethyl Laboratories | Cat #A300-793A | |
| Antibody | Anti-Histone H3 (tri methyl K4) Mouse Monoclonal | Abcam | Cat #ab12209 | |
| Antibody | Anti-Histone H3 (tri methyl K27) antibody Mouse Monoclonal | Abcam | Cat #ab6002 | |
| Antibody | Anti-Histone H3 (acetyl K27) Rabbit Polyclonal | Abcam | Cat #ab4729 | |
| Antibody | Recombinant Anti-Histone H3 (tri methyl K9) Rabbit Monoclonal | Abcam | Cat #ab176916 | |
| Antibody | Anti-Histone H3 antibody Rabbit Polyclonal | Abcam | Cat #ab1791 | |
| Antibody | Anti-ICP4 (58S) Mouse Monoclonal | Neal DeLuca | Hybridomas available from ATCC HB-8183 |