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. 2019 Jul 2;36(11):2415–2431. doi: 10.1093/molbev/msz156

Fig. 3.

Fig. 3.

Fluorescent in situ hybridization (FISH) analysis. Hybridization of both the WBY and Pan32 probes to DAPI-counterstained chimpanzee male chromosomes (A) and bonobo male chromosomes (D), of only the Pan32 probe to DAPI-counterstained chimpanzee male chromosomes (B) and bonobo male chromosomes (E), of only the WBY probe to DAPI-counterstained chimpanzee male chromosomes (C) and bonobo male chromosomes (F). The bonobo Y chromosome is positive for both probes, whereas the chimpanzee Y chromosome is positive only for the WBY probe and not for the Pan32 probe. The Pan32 probe: 5′-amine-modified oligonucleotide probe with a candidate 32-mer male-biased motif sequence is labeled with Alexa Fluor (green). The WBY probe: whole bonobo Y chromosome painting probe is labeled with digoxigenin (red). The white arrows indicate the location of the Y chromosome. Scale bar = 10 μm.