Fig. 4.

(A) Phylogenetic relationship of freshwater sponge species and outgroup taxa, inferred from a concatenated multiple gene alignment using partitioned GTR analysis in MrBayes (3,222 genes, 3,242,264 site alignment, before trimming). This tree was used as the basis for tests of selection on the freshwater lineage, the results of which are summarized in (B) orthogroups displaying significant results for tests of selection under several tests, shown in a Venn diagram indicating consilience of results. (C) Examples of genes and sites under selection, with sites marked according to key at bottom of figure. Domain locations are indicated below each gene as appropriate. Note that further site-level (MEME and BEB) results are available in supplementary file 5, Supplementary Material online.