Figure 2.

OLC8 facilitates GC progression. (A) Efficiency of lentiviral transfection to overexpress OLC8 in AGS and MKN1 cells. (B) The effect of shRNA‐mediated OLC8 silence in GC cell lines. Sh#1 and Sh#2 represented two designed sequences. Since Sh#2 showed higher efficiency, it was selected as ShOLC8. (C) Migration assay for AGS and MKN1 cells transfected with lentiviral control (control), lentiviral vector containing wild‐type OLC8 (OLC8), scramble control (ShCtrl), or shRNA targeting OLC8 (ShOLC8). (D) Quantification results for (C). (E) Viability assay for AGS (top) and MKN1 (bottom) cells. (F) AGS xenograft tumors with OLC8 knockdown or overexpression. (G) Measurements of data from (F). (G) Ki‐67 and immunohistochemical staining for xenograft tumor slides with altered OLC8 expression. Scale bar: 100 µm. (H) Quantification of Ki‐67 results from (G). **P < 0.01