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. 2019 Oct 22;15:357. doi: 10.1186/s12917-019-2099-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Degree of Osteogenic Differentiation in Osteosarcoma Cell Lines. Exposure to osteogenic differentiation media (ODM) demonstrated that 3 of 4 cell lines tested were able to produce a significantly higher number of bone islands than controls. The TOT cell line was surprisingly resistant to the ODM with a significant decrease in the ability to produce bone islands after exposure to ODM. Abrams exceeded the other cell lines in its ability to produce osteoid islands.