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. 2019 Oct 22;20:762. doi: 10.1186/s12864-019-6102-6

Table 6.

Primers used in the qRT-PCR experiment

Primer name Sequence Efficiency (%) R2
ALF1-F 5′-CTG GTG ACG GAA GAA GAA GC-3′ 98.13 0.9975
ALF1-R 5′–CTT AAC CAG GCC ATT CCT CA–3’
Spz-F 5′-CGA CGGA ATA CCC GAC CTA CA-3’ 92.27 0.9926
Spz-R 5′-TGT CGG TTT TGC AGA CGT AG-3’
CuZnSOD3-F 5′-GGG AGA CCT AGG GAA CAT CC-3’ 95.33 0.9920
CuZnSOD3-R 5′-GTG GAT GAC CAC GGC TCT AT-3’
CASP-F 5′-CTG CCC TGA ATT CCT CTC TG-3’ 105.24 0.9885
CASP-R 5′-CGA AGG TGG TAT GGA GCA AT-3’
Anv-F 5′-AAT GGT GGT ATC AGC CTT GC-3’ 94.53 0.9885
Anv-R 5′-TTA GAG GGT CGA CCA TGA GG-3’
DICER-F 5′-CAC TCG AGC ATC CTG TTT CA-3’ 107.80 0.9969
DICER-R 5′-ACC AAT CCC CAT CCA ATG TA-3’
HMCN1-F 5′-TAA GGC AAC CGA CCA CTA CC-3’ 107.85 0.9940
HMCN1-R 5′-GAC GTA GAG ACT GGC GGA AG-3’
ARF-F 5′-CCC ATT ACA GTG GTC CTG CT-3’ 95.22 0.9928
ARF-R 5′-CAG AAC CCT TCC CTT CAC AA-3’
ProPO-F 5′-AAC AAC CTG AGA ACC GGA TG-3’ 93.46 0.9899
ProPO-R 5′-CGG CAG GGT TGG CAT AAT CT-3’
EF1a-F 5′-TGC GCT GTG TTG ATT GTA GC-3’ 103.19 0.9834
EF1a-R 5′-ACA ATG AGC TGC TTG ACA CC-3’