Table 1.
List of primers used for qRT-PCR
| S. No. | Gene name | Primers (from 5′ to 3′) | Product length |
|---|---|---|---|
| 1 | TLR2-A |
Forward 5′-TCGCTCCAACACCTTCGCATTC Reverse 5′-GATTGTCACCGTCGATCCTCAGC |
181 |
| 2 | TLR2-B |
Forward 5′-TCCTCATCCTGGTGGTCGTTGG Reverse 5′-GGCTTCCGCTTGGCTTGGAG |
88 |
| 3 | TLR4 |
Forward 5′-TTCGGTTGGTGGACCTGAATCTTG Reverse 5′-ACAGCTTCTCAGCAGGCAATTCC |
114 |
| 4 | NLRP3 |
Forward 5′-GCTCCTTGCGTGCTCTAAGACC Reverse 5′-TTGTGCTTCCAGATGCCGTCAG |
150 |
| 5 | IL-10 |
Forward 5′-CAGCACCAGTCATCAGCAGAGC Reverse 5′-GCAGGTGAAGAAGCGGTGACAG |
94 |
| 6 | IL-18 |
Forward 5′-AGATGATGAGCTGGAATGCGATGC Reverse 5′-ATCTGGACGAACCACAAGCAACTG |
97 |
| 7 | PTGE |
Forward 5′-GCCTTCTACAGCACGATCCTGATC Reverse 5′-GCCTTCTTCCTGAGCCTCACTTG |
80 |
| 8 | ALOX5 |
Forward 5′-GCGGTTCACAATAGCCATCAACAC Reverse 5′-GCTGTAGGTCAGGTCCTTCATTGC |
139 |
| 9 | ALOX15B |
Forward 5′-GTGAAGGAGCGGACAGTGAAGTG Reverse 5′-AACCAGGCATCCTCCAGGAAGAG |
95 |
| 10 | LOXHD1 |
Forward 5′-CACAGACAAGACCTTCCGCTTCC Reverse 5′-GCAGTCCGTTCCTTCAGTTCCAG |
123 |
| 11 | IFN-γ |
Forward 5′-TTCCTGATGGCGTGAAGAAGGTG Reverse 5′-TCGGAGGATCCACCAGCTTCTG |
129 |
| 12 | GSDMA |
Forward 5′-AGCCTCACAGAAGCCATCTCCTAC Reverse 5′-GCTGCTGCTGCTCGCTGAAG |
196 |
| 13 | GSDME |
Forward 5′-GCTGCGTGCCTGCTCTGATC Reverse 5′-GCTCAGTGCCAAGGTGCCATC |
88 |
| 14 | TRAF2 |
Forward 5′-CGTGGTGATGAAAGGACCCA Reverse 5′-AATGATGTGCTCCCGGTTGT |
100 |
| 15 | TNF-R1 |
Forward 5′-CCTGTCTGTCTTCCCTGTCC Reverse 5′-GGTGCATGGGGTCTTTTCTA |
120 |
| 16 | TRADD |
Forward 5′-CTAGAGCCCAAAGGAAGTCGAT Reverse 5′-TGGCTGCTTCTCTGTGACAT |
100 |
| 17 | FADD |
Forward 5′-GGGGTAAAGAGGCTGAACTCTTA Reverse 5′-TGAGTCCTATTGCACTGCTGTC |
163 |
| 18 | Nrf2 |
Forward 5′-GATGTCACCCTGCCCTTAGA-3′ Reverse 5′-TCGTTCCATTTGTTCCTTCTG-3′ |
124 |
| 19 | GPX-1 |
Forward 5′-AAGTGCTGCTGGTGGTCAACG Reverse 5′-GTTGGTGGCGTTCTCCTGGTG |
155 |
| 20 | GPX-3 |
Forward 5′-GTGGCAGAGGAGTTCGGCAAC Reverse 5′-TCTTGACAGTGGCGATGTTGGC |
151 |
| 21 | PRDX6 |
Forward 5′-GCATCCGCTTCCACGACTTCC Reverse 5′-GGCGTTGATGTCCTTGCTCCAG |
200 |
| 22 | MAP1LC3 |
Forward 5′-GCTGCCAGTGCTGGACAAGAC Reverse 5′-TCCTCATCCTTCTCCTGCTCGTAG |
189 |
| 23 | Beclin-1 |
Forward 5′-ACCGCAAGATTGTGGCTGAAGAC Reverse 5′-TGAGCATAACGCATCTGGTTCTCC |
163 |
| 24 | mTOR |
Forward 5′-AACCACTGCTCGCCACAATGC Reverse 5′-CATAGGATCGCCACACGGATTAGC |
120 |
| 25 | HO-1 |
Forward 5′-TCATTGGCAAGAAGCATCCAGAGC-3′ Reverse 5′-GAACTTGGTGGCGTTGGAGACTC-3′ |
176 |
| 26 | NF-κB |
Forward 5′-CACATGGTGGTGACCGCCAATAG-3′ Reverse 5′-GTGCCATCGTATGTAGTGCTGTCC-3′ |
194 |
| 27 | TNF-α |
Forward 5′-TGATCGTGACACGTCTCTGC-3′ Reverse 5′-CAACCAGCTATGCACCCCAG-3′ |
88 |
| 28 | IL-6 |
Forward 5′-TTCACCGTGTGCGAGAACAGC-3′ Reverse 5′-CAGCCGTCCTCCTCCGTCAC-3′ |
80 |
| 29 | IL-1β |
Forward 5′-AGCAGCCTCAGCGAAGAGACC-3′ Reverse 5′-GTCCACTGTGGTGTGCTCAGAATC-3′ |
90 |
| 30 | Bax |
Forward 5′-ACTCTGCTGCTGCTCTCCTCTC-3′ Reverse 5′-ATCCACGCAGTGCCAGATGTAATC-3′ |
174 |
| 31 | Caspase-3 |
Forward 5′-TACCGGACTGTCATCTCGTTCAGG-3′ Reverse 5′-ACTGCTTCGCTTGCTGTGATCTTC-3′ |
166 |
| 32 | Caspase-8 |
Forward 5′-GGAAGCAGTGCCAGAACTCAGAAG-3′ Reverse 5′-TTGTTGTGGTCCATGCACCGATAG-3′ |
174 |
| 33 | Caspase-9 |
Forward 5′-CCGAAGGAGCAAGCACGACAG-3′ Reverse 5′-CATCTAGCATGTCAGCCAGGTCAC-3′ |
121 |
| 34 | P53 |
Forward 5′-GGAGATGGAACCATTGCTGGAACC-3′ Reverse 5′-GCTCCTGCCAGTTGCTGTGATC-3′ |
113 |
| 35 | Bcl2 |
Forward 5′-GAGTTCGGCGGCGTGATGTG-3′ Reverse 5′-TTCAGGTACTCGGTCATCCAGGTG-3′ |
92 |
| 36 | Cytochrome C |
Forward 5′-CCTAATCGCCGTGGCCTTCTTAAC-3′ Reverse 5′-GGAGGAGGTAGATGGTCGGATTGG-3′ |
163 |
| 37 | NQO1 |
Forward 5′-TCGCCGAGCAGAAGAAGATTGAAG-3′ Reverse 5′-GGTGGTGAGTGACAGCATGGC-3′ |
191 |
| 38 | GSTA2 |
Forward 5′-GGAGTCAATCCGGTGGCTGTTAG-3′ Reverse 5′-GGCTCTGCTCTGCACCATCTTC-3′ |
163 |
| 39 | ATG5 |
Forward 5′-GGACGCATACCAACCTGCTT Reverse 5′-TGCCATTTCAGTGGCGTACC |
200 |
| 40 | Dynein |
Forward 5′-CGTTGCCAGCGTTACACCTATCC Reverse 5′-GCCAGGACTGCCACCAACAC |
163 |
| 41 | F-actin |
Forward 5′-ACCTGGATTGGAGAGGATGTCAGC Reverse 5′-CGGCCTTCTTCAGCTCGTTCTTG |
160 |
| 42 | α-Tubulin |
Forward 5′-GCGGCACGGCAAGTACATGG Reverse 5′-CTTGGTCTTGATGGTGGCGATGG |
94 |
| 43 | TGFβ1 |
Forward 5′-GCCGACACGCAGTACACCAAG Reverse 5′-GCAGGCACGGACCACCATATTG |
168 |
| 44 | β-actin |
Forward 5′-CAACACAGTGCTGTCTGGTGGTAC-3′ Reverse 5′-CTCCTGCTTGCTGATCCACATCTG-3′ |
199 |