Fig. 4.

Inhibition of miR-22 enhanced HCC cell aggressiveness and such enhancement was abolished by treatment with inhibitor of Gal-1. qPCR (a) and western blotting (b) revealed increase of miR-22 and decreased Gal-1 protein expression in stable miR-22 mimic cells, whereas transfection of miR-22 inhibitor in cells resulted in enhanced Gal-1 protein level. c. Anchorage independent growth assays of stable miR-22 mimic and miR-22 inhibitor cells. Stable miR-22 mimic and miR-22 inhibitor cells were subjected to migration (d) and (e) invasion assays. f. The increased number of colonies formed and increase cell migration and invasion was observed in miR-22 inhibitor stable cells compared to control cells was diminished when miR-22 inhibitor cells treated with OTX008 (OTX). Results are expressed as mean +/− SD. P < 0.05 is considered statistically significant