Fig. 3.

The binding of recombinant human L-ficolin to M. avium. a The indicated concentrations of recombinant L-ficolin were incubated with clinically isolated M. avium coated onto microtiter wells. Bound L-ficolin was detected at OD 492 nm. The data show the mean ± SD of three independent experiments. b The binding parameters of recombinant L-ficolin with M. avium were determined by surface plasmon resonance analysis. Sensorgrams for the binding of recombinant L-ficolin to M. avium immobilized on a sensor chip were overlaid at various concentrations of L-ficolin