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. 2019 Oct 21;20:225. doi: 10.1186/s12931-019-1184-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Over-expression of miR-335-5p inhibits NSCLC cell motility and migration and invasion. a-b Transwell assay of the cell migratory and invasive activity in A549 and SPC-A1 cell lines transfected with miR-335-5p mimics. c A wound-healing assay was performed to observe the effect of miR-335-5p transfection in cells. d The expression level of N-cadherin, Vimentin and E-cadherin by quantitative RT-PCR analysis in A549 and SPC-A1 cell lines with miR-335-5p over-expression. e Western blot analysis of Snail, N-cadherin, Vimentin, and MMP2 after transfected with miRNA mimics (miR-335-5p or miR-NC) for 48 h. **P < 0.01; ***P < 0.001