Fig. 2.

Over-expression of miR-335-5p significantly inhibits TGF-β1-induced EMT and cell migration and invasion of NSCLC cells. a-b Transwell assay of A549 and SPC-A1 cells after transfected with miR-335-5p or miR-NC for 24 h, then cells were serum starved for 24 h and treated with or without TGF-β1 (5 ng/ml) for 24 h. c Western blot analysis of the expression of Snail, N-cadherin, Vimentin and MMP2 in A549 and SPC-A1 cells transfected with miR-335-5p mimics or miR-NC in the absence or presence TGF-β1 (5 ng/ml). ***P < 0.001