Table 3.
IHC antibody specifications
| Primary antibody | Use | Retrieval | 2nd antibody | Chromogen | Control |
|---|---|---|---|---|---|
| Monoclonal mouse anti-Ki67 (clone MIB-1; Dako) |
1:50 1 h, RT |
98 °C; pH 9 |
REAL kit (Dako, K5007) |
AEC | Equine skin, rectum |
| Monoclonal mouse anti-p53 (clone DO1; Santa Cruz) |
1:100 1 h, RT |
98 °C; pH 9 | Envision mouse (Dako, K4001) | DAB | Equine SCC |
| Monoclonal mouse anti-MCM7 (clone DCS-141.2; Santa Cruz) |
1:400 1 h, RT |
98 °C; pH 9 | Envision mouse (Dako, K4001) | DAB | Equine lymph node |
| Polyclonal rabbit anti-MMP1 (ThermoFisher) |
1:50 O/N, 4 °C |
98 °C; pH 7 | Envision rabbit (Dako, K4003) | DAB | Equine SCC |
RT Room temperature, O/N overnight, AEC Aminoethylcarbazol, DAB Diaminobenzidine