Table 1.

PCR reaction using single worm lysate

Component	Amount
Single worm lysate	2.0 µl
Forward primer (10 μM)	0.5 µl
Reverse primer (10 μM)	0.5 µl
dNTPs (10 mM each)	0.4 µl
Taq DNA polymerase	0.3 µl (1.5 units)
10× ThermoPol Reaction Buffer	2.5 µl
H2O	18.8 µl