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. 2019 Oct 22;19:978. doi: 10.1186/s12885-019-6210-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Optimal staining for ER, PR and Ki-67 that was deemed to be the reference value. a ER-1: 11–50% positive staining (× 100). b ER-2: > 50% positive staining (× 100). c ER-3: negative staining (× 100). d PR-1: > 50% positive staining (× 100). e PR-2: 1–10% positive staining (× 100). f PR-3: negative staining. g-h KI-1and KI-2: > 30% positive staining in breast cell lines (MCF-7 and MDA-MB-231, × 100). i-k Ki-67 staining in breast carcinoma tissue. i KI-3: 10–30% positive staining (× 100). j KI-4: < 10% positive staining (× 100). k KI-4: > 30% positive staining (× 100). ER: estrogen receptor, PR: progesterone receptor