Fig. 4.

Overexpression of miR-129-5p suppresses the activation of ZIC2-dependent Wnt/β-catenin signaling pathway and EMT in PCa. DU-145 cells were treated with miR-129-5p mimic, miR-129-5p inhibitor or/and si-ZIC2. a The miR-129-5p expression and the mRNA expression of ZIC2, Wnt3a, β-catenin, E-cadherin and vimentin in DU-145 cells determined by RT-qPCR. b, c The protein expression of ZIC2, Wnt3a, β-catenin, E-cadherin and vimentin, and extent of β-catenin phosphorylation in DU-145 cells determined by western blot analysis; *p < 0.05 vs. the blank and NC groups; the experimental data are summarized as mean ± standard deviation; the one-way analysis of variance (ANOVA) was employed to analyze data among groups. This experiment was repeated three times