. 2019 Oct 22;12:495. doi: 10.1186/s13071-019-3742-y

Table 1.

Primers and probes used in this study

Species	Target gene	Primer and probe	Sequence (5′–3′)	T (°C)	Reference
qPCR
Anaplasmataceae	23S rRNA	TtAna-F	TGACAGCGTACCTTTTGCAT	60	[23, 24]
		TtAna-R	GTAACAGGTTCGGTCCTCCA
		TtAna-S	FAM-GGATTAGACCCGAAACCAAG-TAMRA
Conventional PCR
Anaplasma spp.	23S rRNA	Ana23S-212f	GTTGAAAARACTGATGGTATGCA	55	[23, 24]
	23S rRNA	Ana23S-753r	TGCAAAAGGTACGCTGTCAC	55	[23, 24]
	16S rRNA	AENW-16S-F1	GCAGACGGGTGMGTAAYG	50	[73]
		AENW-16S-R	GTGCCAGCAGCCGCGGTAAT
		AENW-16S-F2 (seq.)	GTGCCAGCAGCCGCGGTAAT
	rpoB	Ana-rpoBF	GCTGTTCCTAGGCTYTCTTACGCGA	55	[22]
	rpoB	Ana-rpoBR	AATCRAGCCAVGAGCCCCTRTAWGG	55	[22]
Ehrlichia spp.	groEL	Ehr-groEL-F	GTTGAAAARACTGATGGTATGCA	50	[22]
Ehrlichia spp.	groEL	Ehr-groEL-R	ACACGRTCTTTACGYTCYTTAAC	50	[22]
Ticks	12S rDNA	T1B	AAACTAGGATTAGATACCCT	51	[21]
Ticks	12S rDNA	T2A	AATGAGAGCGACGGGCGATGT	51	[21]

Abbreviation: T, annealing temperature; seq., sequencing