Fig. 4.

HOTAIR directly interacts with miR-326. a Cytoplasmic and nuclear levels of HOTAIR in A431 and SCL-1 analyzed by qRT-PCR. b Bioinformatics predictions from RegRNA and Starbase. c MiR-326 expression in CSCC cell lines and HaCaT detected by qRT-PCR. d Binding of miR-326 onto 3′-UTR of HOTAIR evidenced by bioinformatics. e Dual-luciferase reporter gene assay in A431 and SCL-1 after transfection with NC or miR-326 mimics, Renilla luciferase plasmid pRL-SV40 and the reporter constructs. f Detection of the amount of HOTAIR and miR-326 in A431 and SCL-1 via RIP experiments. Data are presented as mean ± SD. *P < 0.05