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. 2019 Oct 21;19:270. doi: 10.1186/s12935-019-0992-x

Fig. 5.

Fig. 5

PRAF2 is the direct target of miR-326. a The putative miRNA binding sites in the PRAF2 sequence. b Dual-luciferase reporter gene assay is carried out to verify the direct target sites. c PRAF2 expression in CSCC cell lines and HaCaT detected by qRT-PCR. d Protein levels of PRAF2 in HaCaT, A431 and SCL-1 are detected by Western blotting. Data are presented as mean ± SD. *P < 0.05