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. 2019 Oct 16;7:243. doi: 10.3389/fcell.2019.00243

FIGURE 4.

FIGURE 4

MRTF-A interacts with Sp1 to activate Abl1 transcription in hepatic stellate cells. (A) LX-2 cells were treated with or without PDGF-BB for 24 h. Re-ChIP assay was performed with indicated antibodies. (B) Primary mouse HSCs were isolated and spontaneously activated in culture for 7 days. Re-ChIP assay was performed with indicated antibodies. (C) An Abl1 promoter-luciferase construct was transfected into LX-2 cells with indicated expression constructs. Luciferase activities were normalized by both protein concentration and GFP fluorescence. (D) Wild type or mutant Abl1 promoter-luciferase constructs were transfected into LX-2 cells with or without MRTF-A. Luciferase activities were normalized by both protein concentration and GFP fluorescence. (E,F) LX-2 cells were transfected with small interfering RNA targeting Sp1 or SCR followed by treatment with PDGF-BB. Knockdown efficiencies were examined by Western. ChIP assays were performed with anti-MRTF-A. (G) LX-2 cells were with PDGF-BB and/or mithramycin A for 24 h. ChIP assays were performed with anti-MRTF-A. All experiments were repeated three times and one representative experiment is shown. Error bars represent SD (∗p < 0.05, one-way ANOVA with post hoc Scheffe test).