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. 2019 Aug 5;30(10):2041–2050. doi: 10.1007/s13361-019-02265-9

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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(a) Molecular images of the brain highlighted in red; the regions of interest (ROIs) delineated for the analysis: prelimbic cortex (pc), caudate putamen (cp), hippocampus (h), white (cwm) and gray (cgm) matter in the cerebellum. (b) 3D loading plot for the pLSA of the whole brains of the three groups. Blue represents the control group (C), green the hypercholesterolemic control (HC), and red the hypercholesterolemic treated group (HT). Results display no separation among the different groups. (c) Molecular images based on the distribution of cholesterol sulfate (m/z 465.3) over the whole brain for the C, HC, and HT groups. (d1, d2) ROC plots of the m/z 465.3 (cholesterol sulfate) from the comparison between (d1) C and HC, and between (d2) HT and HC