Fig. 3.

Comparison of the sensory switch circuit with the other switch circuits in vitro and in vivo. a Circuits performance in response to leaky expression of Gal4VP16 in vitro. Circuits were co-transfected along with varying amount of the CAG-driven Gal4VP16 (LK plasmid) as leaky expression into HEK293 cells. Each data point shows mean ± s.d. from three independent replicates, *P < 0.05, **P < 0.01, ***P < 0.001. b Schematic diagram of in vivo experimental design with xenografted HepG2 nude mouse model. c 1 × 10⁹ VP of OV-Circuits were injected into xenografted tumor twice a week right after the size of tumor reached ~100 mm³. PBS was used as a negative control. The left panel shows the mouse weight after treatment. Each data point shows the mean ± s.d. weight (n = 10) at indicated day. The right panel shows the xenografted tumor size after treated. Each data point shows the mean ± s.d. tumor size (n = 10) at indicated day, *P < 0.05, **P < 0.01, ***P < 0.001,  Student’s t-test was performed. Source data are provided as a Source Data file