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. 2019 Oct 22;10:4801. doi: 10.1038/s41467-019-12794-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Functional assay of synthetic oncolytic adenovirus in vitro. a The schematic diagram of adenoviral genome loaded with sensory switch circuit encoding different immune effectors (OV-Effector). The survival ratios of different human HCC cells in Panel b and mouse Hepa1-6 cells in Panel c were measured by using MTS cell viability assay 6 days after incubation with indicated amount of OV-EBFP. Data are shown as mean ± s.d. from three independent replicates. d–f 3 × 10⁶ HepG2 cells were incubated with 10 MOI of oncolytic adenovirus encoding indicated immune effectors for 4 days and the supernatants were collected for measurement of mGM-CSF production by using ELISA in (d), or the bioactivity of IL-2 by using MTS proliferation assay on cytokine-dependent cell line CTLL-2 in (e), or the bioactivity of anti-PD-1-scFv and anti-PD-L1-scFv by using ELISA in mouse splenocyte system in the presence of anti-CD3 antibody in (f). Data are shown as mean ± s.d. from three independent replicates. Source data are provided as a Source Data file