Fig. 5.

Effect of synthetic oncolytic adenovirus in nude mouse models. a The schematic diagram of OV-EBFP and non-replicable adenovirus (Ad-GFP). The experimental design of nude mouse experiments is shown in the right panel. b 1 × 10⁹ VP of OV-EBFP or Ad-GFP were injected into indicated xenografted tumor twice a week right after the size of tumor reached ~100 mm³. PBS was used as a negative control. Each data point shows the mean ± s.d. tumor size (n = 9 or 10) at indicated day. c DNA (left) and RNA (right) distribution of OV-EBFP in HepG2 xenografted nude mice 1 week after injection of 1 × 10⁹ VP of OV-EBFP (n = 7 in left, n = 5 in right) when the size of tumor exceeded ~500 mm³. PBS (n = 4) was used as a negative control. Data are shown as mean ± s.d. from three independent replicates by using quantitative PCR. Student’s t-test was performed. Data are shown as mean ± s.d., *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file