Fig. 7. Knockdown of IRE1 or ATG7 expression reverses α-Synuclein-induced neurodegeneration.
a Lifespan of Ddc-Gal4 > +, Ddc-Gal4 > α-SynA30P, Ddc-Gal4 > α-SynA30P; Ire1-Ri, and Ddc-Gal4 > α-SynA30P; Atg7-Ri flies (n = 90 flies/genotype). b Climbing ability of the indicated genotype at 1, 3, or 5 weeks of age. Relative activities are shown as mean ± s.e.m. (n = 90 flies/genotype; two independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA. c Representative Z-stack confocal images of whole brains stained with anti-TH antibody for the indicated lines at 40 days of age. TH-positive DA neurons of the indicated clusters were quantified. PAL protocerebral anterior lateral, PPM protocerebral posterior medial, PPL protocerebral posterior lateral. The data are shown as mean ± s.e.m. (n = 5 flies/genotype; two independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA. Scale bar represents 100 µm