Fig. 5.

NEE blocks the multigenerational transmission of HFD-dependent cognitive impairment. a Latency to reach the hidden platform in the MWM test for SD, F2_SD NEE, F2_HFD, and F2_HFD NEE mice (see Fig. 1 for the experimental design; n = 8 mice derived from 6 litters for each group; significance is indicated between F2_HFD and F2_HFD NEE mice; statistics by two-way ANOVA and Bonferroni post hoc). b Time spent in the four quadrants during the probe test of MWM by SD, F2_SD NEE, F2_HFD, and F2_HFD NEE mice. NE is the target quadrant (n = 8 mice derived from 6 litters for each group; statistics by two-way ANOVA and Bonferroni post hoc). c Bar graphs showing changes in fEPSP amplitudes and d slopes in SD, F2_SD NEE, F2_HFD, and F2_HFD NEE mice (n = 11 slices from 4 mice of different litters per each group; statistics by two-way ANOVA and Bonferroni post hoc). e Bdnf exon I, IV, and IXa expression (normalized to actin) in the hippocampus of SD, F2_SD NEE, F2_HFD, and F2_HFD NEE mice. Data represent mean values obtained from six mice derived from five litters for each group; experiments were performed in triplicate (statistics by two-way ANOVA and Bonferroni post hoc). f BDNF levels in the hippocampus of SD, F2_SD NEE, F2_HFD, and F2_HFD NEE mice. ELISA assay was performed in duplicate (n = 8 mice derived from 5 litters per group; statistics by two-way ANOVA and Bonferroni post hoc). g ChIP assays of H3K9ac and H3K4me3 on the promoters I, IV, and IX of Bdnf gene in the hippocampus of SD, F2_SD NEE, F2_HFD, and F2_HFD NEE mice. Data represent mean values obtained from 6 mice derived from 4 to 5 litters for each group; qPCR experiments were performed in triplicate (statistics by two-way ANOVA and Bonferroni post hoc). Data are expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; n.s. not significant