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. 2019 Oct 22;10:4804. doi: 10.1038/s41467-019-12544-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Rasal1 KD T cells show enhanced rejection of B16 tumors and the increased presence of activated CD8 + TILs. a Schematic of B16 melanomas tumor experiment. Histogram of B16 tumor foci at day 14 in control versus or mice receiving OT-1 cells with control scrambled esiRNA or with Rasal1 esiRNA (n = 4 per group, upper panel). Lower panel: representative image of B16 tumor lungs from each group. Right panel: histogram showing Rasal1 KD reduction of tumor foci in pulmonary B16 metastasis (numbers of tumor foci in lungs) (n = 3) (scale bar, 10 mm). b Rasal1 KD adoptive cell transfer results in a major increase in CD8 + TILs in B16 tumors. Left panel: FACs histograms showing presence of CD8 + TILs in tumors. SC scrambled siRNA, Rasal1 KD Rasal1 siRNA. Right upper panel: histogram showing percentage of CD8⁺ TILS; lower right panel: percentage (%) of CD4 vs CD8 T-cells in lung tumors of B16 animals. c Rasal1 KD ACT results in an increase in the GZMB and interferon gamma expressing CD8 + TILs in B16 tumors. Histograms showing % of granzyme B (GZMB), IFN-γ, IL-2, and TNF-α expressing CD8 + TILs. Plots show mean with ± SD. p-values were calculated by unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001