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. 2019 Oct 22;9:15156. doi: 10.1038/s41598-019-51659-y

Table 2.

Multilevel model analysis of the association between individual and walkability and lung function, pH, exhaled NO and pupillometry parameters explained by school.

Outcome Walkabilityβ (95% CI) School
ICC Variance Explained variation*
FVC
Model 0 −0.58 (−2.79; 1.63)
Model 1 1.78% 4.48 Reference
Model 5a −2.62 (−6.00; 0.77) 1.32E-6% 2.51E-6 >99.9%
FEV 1
Model 0 −1.02 (−3.07; 1.02)
Model 1 2.09% 4.53 Reference
Model 5a −2.63 (−5.71; 0.46) 4.47E-7% 7.09E-7 >99.9%
FEF 25%-75%
Model 0 −1.27 (−4.74; 1.98)
Model 1 0.37% 2.14 Reference
Model 5a −0.72 (−6.33; 4.89) 5.27E-7 2.77E-6 >99.9%
EBC pH
Model 0 0.09 (−0.03; 0.21)
Model 1 3.48% 0.03 Reference
Model 5a −0.004 (−0.34; 0.33) 8.52% 0.08 >−99.9%
Exhaled NO
Model 0 −0.05 (−0.17; 0.07)
Model 1 3.98% 0.03 Reference
Model 5b −0.07 (−0.38; 0.23) 8.07% 0.06 −90.6%
Baseline pupil diameter
Model 0 −0.22 (−0.34; −0.09)
Model 1 20.4% 0.158 Reference
Model 5c −0.18 (−0.54; 0.17) 22.1% 0.178 −12.5%
Final pupil diameter
Model 0 −0.04 (−0.13; 0.05)
Model 1 14.1% 0.053 Reference
Model 5c −0.05 (−0.26; 0.16) 15.1% 0.059 −10.3%
ACV
Model 0 −0.22 (−0.32; −0.12)
Model 1 14.7% 0.070 Reference
Model 5c −0.21 (−0.46; 0.03) 16.7% 0.081 −16.4%
MCV
Model 0 −0.31 (−0.45; −0.17)
Model 1 12.3% 0.112 Reference
Model 5c −0.29 (−0.60; 0.02) 10.1% 0.091 18.0%
Constriction amplitude
Model 0 −1.94 (−2.65; −1.23)
Model 1 8.38% 2.073 Reference
Model 5c −1.62 (−2.87; −0.37) 7.52% 1.847 10.9%
ADV
Model 0 0.01 (−0.04; 0.06)
Model 1 8.57E-8% 8.10E-11 Reference
Model 5c 0.005 (−0.06; 0.07) 7.82E-8% 7.48E-11 7.60%
T75
Model 0 −0.17 (−0.29; −0.06)
Model 1 4.53% 0.023 Reference
Model 5c −0.19 (−0.36; −0.02) 3.19% 0.016 29.3%

*corresponds to the proportion of between-schools variance that could be explained by exposure and individual characteristics; 95% CI: 95% confidence interval; ICC: intra-class correlation coefficient; FVC: forced vital capacity; FEV1: forced expiratory volume in the first second of FVC; FEF25–75: forced expiratory flow in the middle portion of FVC; EBC: Exhaled breath condensate; ACV: Average constriction velocity; MCV: Maximum constriction velocity; ADV: Average dilation velocity; T75: the total time taken by the pupil to recover 75% of its initial resting diameter after it reached the peak of constriction.

Model 0 only included the PC1 and PC2 score; a Model 1 is null model, baseline model without any exposure variable; Model 5a is additionally adjusted for age, sex, asthma, WHO z-score for BMI and family history of asthma or allergy; Model 5a is additionally adjusted for age, sex, asthma, atopy, WHO z-score for BMI and family history of asthma or allergy; Model 5c is additionally adjusted for age, sex, asthma, and WHO z-score for BMI.