Table 1.
Relative amounts of SSV1 virus DNA of silenced and control cultures infected at different MOIs
| MOI, n | V-DNA found in | PI (min) | SB3×6 | Ctrl | Sig. |
|---|---|---|---|---|---|
| MOI: 0.1 n = 3 | Supernatant | 30 | 112 (±13) | 87 (±10) | ** |
| 60 | 101 (±30) | 59a (±11) | * | ||
| Cell | 30 | 0.80 (±0.22) | 4.44 (±2.82) | ** | |
| 60 | 3.08 (±0.64) | 11.99 (±6.12) | ** | ||
| MOI: 5 n = 3 | Supernatant | 35 | 75 (±20) | 43 (±11) | |
| 50 | 43 (±3) | 24 (±8) | * | ||
| Cell | 35 | 0.97 (±0.17) | 1.60 (±0.28) | *** | |
| 50 | 0.97 (±0.38) | 1.45 (±0.30) | * | ||
| MOI: > 100 n = 3 | Supernatant | 30 | 71 (±14) | 59 (±6) | * |
| 60 | 63 (±6) | 43 (±7) | *** | ||
| Cell | 30 | 1.66 (±0.23) | 1.85 (±0.28) | ||
| 60 | 2.14 (±0.13) | 2.00 (±0.19) |
Extracellular (supernatant) and intracellular (cell) virus amounts of same cultures were measured in relation to timepoint 10 min PI as percentage and fold change, respectively. Measurements of the same cultures (three biol. repl. each) at two different timepoints postinfection (PI) are presented. Significant differences (column Sig.) between SB3×6 and Ctrl at the same timepoint are indicated with asterisks, where p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***) (two-tailed t test, n ≥ 3)70
Bold values indicate significant differences of respective timepoints to timepoint 10 min PI in each individual culture (p ≤ 0.009 for extracellular; p ≤ 0.05 for intracellular)