FIG 3.
Interactome of the nitrate reductase in the hcp-deficient strain. (A) Volcano plot of proteins immunoprecipitated with NarGeGFP versus untagged version of NarG under nitrate-respiring conditions. The volcano plot was constructed and displayed as described in the legend to Fig. 2. Black dots located outside the curves represent proteins differentially found between the two conditions with q values below 1% (Table S4). Proteins more enriched using the hcp strain are circled in red. (B) The two NO reductases, Hmp and NorV with its redox partner NorW, and YtfE (RIC) protein were found more abundantly associated with the nitrate reductase in the hcp strain. The heat map shows the Z-scored LFQ intensities obtained for each biological replicate in the wild-type strain (left) and in the hcp strain (right) grown under nitrate-respiring conditions according to the color gradient below.