FIG 4.

Inactivation of nirB and hcp induces a growth defect under nitrate-respiring conditions caused by elevated nitrosative stress. Generation times of strains JCB4011 (WT), LCB4136 (hcp), LCB4121 (nirB), LCB4137 (nirB hcp) grown under anaerobiosis in defined medium with glycerol as sole carbon source and nitrate as the sole electron acceptor without Casamino Acids (A) or supplemented with BCAA (B) are shown. Eight (A) and four (B) independent assays are, respectively, shown. Medians are represented by a horizontal line and averages by a red square. Statistical significance between the different strains was calculated using the Mann-Whitney nonparametric test: ***, P < 0.001; **, 0.001 < P < 0.01; ns, not significant (P > 0.05).