TABLE 2.
Mutations occurring in animal POST1 under oseltamivir treatmenta
| Tissue | Analysis | % of sequence showing mutations (no. of plaques showing mutations/total no. of plaques analyzed) |
||||
|---|---|---|---|---|---|---|
| NA His275Tyr |
HA Asp187Val |
HA Asp187Asn |
HA Asp222Asn |
HA Asp222Gly |
||
| Nasal mucosa | Plaque | 100 (20/20) | 0 (0/20) | NT | NT | NT |
| Trachea | PCR | 100 (5/5) | 0 (wt) | 0 (wt) | 75 (3/4) | 25 (1/4) |
| Right bronchus | PCR | 100 (2/2) | 75 (3/4) | 0 (wt) | 25 (1/4) | 0 (wt) |
| Left bronchus | PCR | 100 (3/3) | 75 (wt) (3/4) | 25 (1/4) | 25 (1/4) | 75 (wt) (3/4) |
| Right lung upper | PCR | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) |
| Right lung middle | PCR | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) |
| Right lung caudal | Plaque | 100 (20/20) | 85 (17/20) | 15 (3/20) | 0 (wt) | 0 (wt) |
| Left lung upper | PCR | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) |
| Left lung middle | PCR | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) |
| Left lung caudal | PCR | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) | 0 (wt) |
a
Plaque, virus isolation by plaque assay and subsequent direct sequence determination; PCR, RT-PCR of total RNA isolated from the specimen followed by molecular cloning and sequence determination (2 to 6 individual clones each); NT, not tested; wt, wild-type sequence.