FIG 1.

SRPK1 binds to the RGG box of ICP27 in vitro. (A) Schematic of subdomains and interaction sites within HSV-1 ICP27. (B) Affinity quantification of SRPK1 interaction with unmodified ICP27^103–155. (C) As in panel B but with methylated ICP27^103–155. (D) ¹H-¹⁵N correlation spectra of [¹⁵N]ICP27^103–155 in the absence (red) and presence (blue) of a stoichiometric equivalent of SRPK1. (E) Binding site mapped by analysis of relative signal intensity change upon binding, I^B/I^F. The decrease in signal intensity of a protein occurs upon interaction with SPRK1.