Figure 1. RA-induced differentiation of SH-SY5Y cells. Cells were treated with RA (50 µM) for 5 and 10 d. (a) After RA treatment, the expression of tyrosine hydroxylase (TH) was visualized by immunostaining in undifferentiated cells and cells differentiated with RA for 5 or 10 d. (b) TH protein was detected by Western blotting. β-actin was used as a normalization control. The density of bands was analyzed based on the expression of β-actin. Data are expressed as mean ± SD from three independent experiments. *** P < 0.001 versus the undifferentiated group.
