Figure 3. The lncRNA RPPH1 and miR-330-5p have a targeting effect relationship in THP-1 cells. (A) The Venn diagram shows the candidate miRNAs that were predicted to be targeted by the lncRNA RPPH1, which were revealed by two tools starBase v 2.0 and lncbase. Schematic diagram shows the matching base pairs of RPPH1 and miR-330-5p. (B) The relative expression level of indicated miRNAs in the control stable THP-1 cell line (sh-NC) and stable RPPH-knockdown THP-1 cell line (sh-RPPH1) was determined with RT-qPCR. (C)The luciferase activity of the reporter vectors was detected in 293T cells after 48 hour of co-transfection of wild type (RPPH1-wt) or mutant (RPPH1-mut) RPPH1 3'UTR with miR-330-5p mimics. (D) The relative expression level of miR-330-5p in human bone marrow stromal cell line HS-5 and three human AML cell lines (THP-1, HL-60 and KG-1) was determined with RT-qPCR. *P<0.05, **P<0.01; compared with the indicated controls.