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. 2019 May 10;160(11):2737–2747. doi: 10.1210/en.2019-00100

Figure 4.

Figure 4.

NNC0165-1273 blocks the feeding and cFos responses to ghrelin to a similar extent as PYY3-36. Preadministration of (A) NN (0.04 µmol/kg) or (B) PYY3-36 (0.012 μmol/kg) abrogated the feeding induced by a daytime injection of ghrelin (2 mg/kg), as well as the induction of cFos in NPY/AgRP neurons of the hypothalamic arcuate nucleus. Representative photomicrographs of fluorescent Npy-hrGFP neurons following injection of (Ci) vehicle, (Cii) ghrelin, (Ciii) NN, or (Civ) NN + ghrelin. (D) The percentage of NPY/Agrp neurons containing cFos following the same injections as in (A) and (B). Data presented as mean ± SEM. Two-way ANOVA with (A, B) Sidak post hoc tests: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; one-way ANOVA with (D) Sidak post hoc test: *P < 0.05, ****P < 0.0001.