Fig. 2.

Effect of tocopherols on bulk fluid-phase uptake in diseased endothelial cells. (A) Fluorescence microscopy of control (Ctrl) and imipramine-diseased endothelial cells (Dis) incubated for 1-hour pulse with Texas Red dextran, 1 hour after treating cells for 48 hours with 40 µM δ-tocopherol or 20 µM CF3-T. Dotted lines mark the cell borders, observed by phase-contrast microscopy. Scale bar, 10 µm. (B) Dextran uptake was quantified per cell and normalized to untreated diseased cells (horizontal solid line). Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated control cells; ^†comparison with untreated diseased cells (P < 0.05 by Student’s t test).