Fig. 2.

Complexation and protection of siRNA from RNase. (A) Agarose gel electrophoresis of polyplex with different N/P w/w ratios. Uncomplexed siRNA is shown as white bands on the gel. Complete complexation took place at N/P w/w ratios of 1/4 and 1/6 w/w. (B) siRNA degradation assay showed no increase in absorbance (a measurement of free siRNA) in 1/4 P(SiDAAr)₅PEG₃ polyplex, indicating polymer protecting siRNA from RNase degradation. Data are the mean ± S.E.M.; n = 2 to 3. P < 0.01 between siRNA, siRNA-polyplex + nuclease vs. siRNA + nuclease.